Clinical Trial: Characterization of Immune Semaphorin in Non Alcoholic Fatty Liver Disease and NASH

Study Status: Recruiting
Recruit Status: Recruiting
Study Type: Interventional

Official Title: Characterization of Immune Semaphorin in Non Alcoholic Fatty Liver Disease and NASH

Brief Summary:

Recent epidemiological studies in France showed a high prevalence of obesity (14.5%) and its strong increase in the last 20 years. Among the many complications associated with obesity, liver complications (steatosis and steatohepatitis [NASH]) are among the most common. Semaphorins were described in the early 1990. More than 20 types of these proteins have been reported to date. These proteins were used for neural development. Since many functions have also been described. The semaphorins are involved in numerous physiological or physiopathological processes (cardiac morphogenesis, vascular growth, tumor progression), the regulation of immune cells and liver fibrosis. Preliminary studies have allowed to show that dendritic cells infiltrate adipose tissue and initiate the activation of T cells and inflammation. Immune semaphorin are new players in the regulation of inflammation and immune reactions.

The role of immune semaphorin in regulating inflammation in the two compartments (liver and adipose tissue) could be a crucial step that could lead to more severe liver damage. Its dysregulation could explain NASH injuries. The goal is to identify a new mode of regulation of cellular homeostasis in the fatty liver disease. These factors may serve as diagnostic markers or future therapeutic targets.


Detailed Summary:
Sponsor: Centre Hospitalier Universitaire de Nice

Current Primary Outcome:

  • Evaluation of the staging of liver fibrosis [ Time Frame: Day 0 ]
    The investigators determine the stage of liver fibrosis by histological study of biopsies
  • Determination of the expression of level of semaphorin [ Time Frame: Day 0 ]
    The investigators determine by technic of Reverse Transcription Polymerase Chain Reaction (RT-PCR) the expression of level of semaphorin
  • Determination of the composition of immunity cells [ Time Frame: Day 0 ]
    The investigators determine the composition of immunity cells by Immunohistochemical and biochemical analyses (Western Blotting)


Original Primary Outcome: Same as current

Current Secondary Outcome:

Original Secondary Outcome:

Information By: Centre Hospitalier Universitaire de Nice

Dates:
Date Received: June 20, 2016
Date Started: March 2013
Date Completion: September 2016
Last Updated: June 28, 2016
Last Verified: June 2016