Clinical Trial: Fat Cell Size and Overfeeding and Etopic Study

Study Status: Active, not recruiting
Recruit Status: Active, not recruiting
Study Type: Interventional

Official Title: Fat Cell Size and Overfeeding and Etopic Study

Brief Summary: It has been shown that large fat cells are associated with more risks for insulin resistance and more cardiovascular risk factors such as high cholesterol or triglycerides.

Detailed Summary: This study will look at fat cell size and how it affects fat deposition, insulin resistance and muscle oxidative capacity. A comparison will be made with the differences between participants with larger versus smaller fat cells in insulin resistance and blood levels of lipids. The study will also determine if characteristics of fat cells and muscle can predict the development of weight gain-induced insulin resistance.
Sponsor: Pennington Biomedical Research Center

Current Primary Outcome: Determine the effect of 8-weeks 40% high fat overfeeding in 40 healthy, young adults on adipose and skeletal muscle characteristics, ectopic fat accumulation, insulin sensitivity, and metabolic flexibility. [ Time Frame: 10 months ]

This study will investigate how fat cell size (independent of total fat mass) affects metabolic outcomes of overfeeding, including changes in fat deposition, insulin sensitivity and muscle oxidative capacity. It has been shown that large fat cells relative to fat mass are associated with higher risks for insulin resistance and type 2 diabetes.


Original Primary Outcome: Same as current

Current Secondary Outcome:

  • Fat Deposition [ Time Frame: 10 Months ]
    Viewing of adipose tissue or body fat and skeletal muscle characteristics over a period.
  • Insulin Sensitivity [ Time Frame: 10 months ]
    Measure and monitor the natural hormone insulin becomes less effective at lowering blood sugars. The resulting increase in blood glucose may raise levels outside the normal range and cause adverse heealth effects, depending on dietary conditions or sensitivity of cells to insulin.
  • Muscle Oxidative Capacity [ Time Frame: 10 Months ]
    A measure of a muscle's maximal capacity to use oxygen in microliters of oxygen consumed per gram of muscle per hour.
  • Circadian Blood Pressure variability [ Time Frame: 10 Months ]
    Blood pressure and heart rate measurement: An automatic BP monitoring device (Mortara® Ambulo 2400) for ambulatory use was attached to a BP cuff to obtain blood pressure (BP) and heart rate (HR) readings at 30-min intervals during the day (6:30 AM to 9:30 PM) and 60-min intervals at night (10 PM to 6 AM) while the participants went about their activities. Data were downloaded into the database at the end of the 7-day recording span for a chronobiological analysis. This was done at baseline, post-overfeeding and at 3 months.
  • Resting Endothelial function testing [ Time Frame: 10 Months ]
    Endothelial function testing: Assessment of resting endothelial function was done with the participant in a fasting state, after having avoided stimulants (caffeine, tobacco, alcohol, exercise) for 12 hours, at the same fixed clock hour (range 8-10 AM), using the EndoPAT 2000 device manufactured by ITAMAR Medical®.


Original Secondary Outcome: Same as current

Information By: Pennington Biomedical Research Center

Dates:
Date Received: August 20, 2012
Date Started: May 2008
Date Completion: May 2017
Last Updated: September 26, 2016
Last Verified: September 2016