Clinical Trial: Gene Polymorphism Associated With Macroangiopathy in Type 2 Diabetes Patients
Study Status: Completed
Recruit Status: Completed
Study Type: Observational
Official Title: Polymorphism Analysis of HLA-DRB1*04 Alleles in Patients With Type 2 Diabetic Macroangiopathy in Northeast Chinese
Brief Summary: To explore the possible implications of HLA-DRB1*04 alleles in patients with type 2 DM and macroangiopathy
Detailed Summary:
Previous studies examining the molecular etiology of diabetes mellitus in China and abroad have mainly focused on the relationship between HLA and type 1 diabetes mellitus (DM) (1-3). Due to the complexity of type 2 DM heredity, few researchers have studied the correlation between type 2 DM and HLA. In recent years, it has been suggested that the development of coronary heart disease (CHD) in diabetic individuals is not merely a complication of diabetic macroangiopathy. CHD and diabetic macroangiopathy share some genetic associations. Previous studies have demonstrated that certain HLA*DRB1*04 subtypes are associated with an increased risk of cardiovascular disease.
Experimental methods: For genomic DNA extraction, 3-5ml venous blood samples were collected from all patients. Coagulation was prevented using EDTA.
The procedure for PCR amplification was as follows: denaturing for 100s at 96°C, annealing for 60s at 63°C, 1 cycle; denaturing for 10s at 96°C, annealing for 60s at 63°C, 9 cycles; denaturing for 10 s at 96°C, annealing for 30 s at 59°C; extending for 30 s at 72°C, 20 cycles; maintaining at 4°C. Analyzing PCR products stained by Ethidium Bromide on 2.5% agarose gel electrophoresis. Bands were observed using an ultraviolet light and a biological imaging system.
Statistical method: The degree of agreement with Hardy-Weinberg equilibrium was determined using the χ2 test. A t-test was employed for comparison of CRP levels (M ± s) and a χ2 test was performed to compare allelic frequencies. The relative risk rate (RR) was calculated as (Number of patients with the gene×Number of people in control group without the gene) divided by (Number of people in control group with the gene×Number of the patients witho
Sponsor: First Affiliated Hospital of Harbin Medical University
Current Primary Outcome: Test of allelic frequencies [ Time Frame: At recruitment ]
Original Primary Outcome: Same as current
Current Secondary Outcome: Evaluation of serum CRP(C-reactive protein) levels [ Time Frame: At recruitment ]
Original Secondary Outcome: Same as current
Information By: First Affiliated Hospital of Harbin Medical University
Dates:
Date Received: August 25, 2016
Date Started: May 2015
Date Completion:
Last Updated: August 25, 2016
Last Verified: August 2016