Clinical Trial: Drug Transport in Patients With Anorexia Nervosa

Study Status: Completed
Recruit Status: Unknown status
Study Type: Observational

Official Title: Influence of Cholesterol Plasma Levels on Drug Transport in Patients With Anorexia Nervosa

Brief Summary: The aim of this study is to investigate whether patients with anorexia nervosa have elevated plasma cholesterol levels and, if elevated plasma cholesterol levels influence drug transport by the ABC-transporter P-gp (P-glycoprotein, MDR 1/ABCB1). We will isolate peripheral blood mononuclear cells (PBMCs) and total RNA from the blood of patients with anorexia nervosa and healthy subjects as control. PBMCs will be used for quantitative cholesterol determination and for measuring the activity of P-gp using a FACS (fluorescence-activated cell sorting) method (rhodamine123 efflux). Total RNA will be used for quantitative expression analysis of P-gp by reverse transcriptase real time PCR.

Detailed Summary:
Sponsor: Heidelberg University

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Information By: Heidelberg University

Dates:
Date Received: September 13, 2005
Date Started:
Date Completion:
Last Updated: September 13, 2005
Last Verified: April 2003